FEBS Letters | |
YDL142c encodes cardiolipin synthase (Cls1p) and is non‐essential for aerobic growth of Saccharomyces cerevisiae | |
Klein, Franz1  Hrastnik, Claudia2  Achleitner, Georg2  Schiefthaler, Ursula1  Tuller, Gabriele2  Daum, Günther2  | |
[1] Institut für Botanik, Universität Wien, Rennweg 14, A-1030 Wien, Austria;Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Petersgasse 12/2, A-8010 Graz, Austria | |
关键词: Cardiolipin; Phosphatidylglycerol; Phosphatidylglycerolphosphate; Mitochondria; Cytochrome c oxidase; Yeast; | |
DOI : 10.1016/S0014-5793(97)01525-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid of cardiolipin but accumulate the cardiolipin precursor phosphatidylglycerol when grown on non-fermentable carbon sources. Specific activity of phosphatidylglycerolphosphate synthase in cls1 is reduced to 30–75% of the wild-type level. Amounts of mitochondrial cytochromes and activity of cytochrome c oxidase, however, are not affected in the cls1 deletion strain. Collectively, these data indicate that cardiolipin is not essential for aerobic growth of Saccharomyces cerevisiae.
【 授权许可】
Unknown
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