YDL142c encodes cardiolipin synthase (Cls1p) and is non‐essential for aerobic growth of Saccharomyces cerevisiae

FEBS Letters

Klein, Franz¹  Hrastnik, Claudia²  Achleitner, Georg²  Schiefthaler, Ursula¹  Tuller, Gabriele²  Daum, Günther² 

[1] Institut für Botanik, Universität Wien, Rennweg 14, A-1030 Wien, Austria;Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Petersgasse 12/2, A-8010 Graz, Austria

关键词: Cardiolipin;    Phosphatidylglycerol;    Phosphatidylglycerolphosphate;    Mitochondria;    Cytochrome c oxidase;    Yeast;   

DOI  :  10.1016/S0014-5793(97)01525-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid of cardiolipin but accumulate the cardiolipin precursor phosphatidylglycerol when grown on non-fermentable carbon sources. Specific activity of phosphatidylglycerolphosphate synthase in cls1 is reduced to 30–75% of the wild-type level. Amounts of mitochondrial cytochromes and activity of cytochrome c oxidase, however, are not affected in the cls1 deletion strain. Collectively, these data indicate that cardiolipin is not essential for aerobic growth of Saccharomyces cerevisiae.

【 授权许可】

Unknown