Functional expression of the murine D2, D3 and D4 dopamine receptors in Xenopus laevis oocytes

FEBS Letters

Levavi-Sivan, Berta¹  Fuchs, Sara¹  Skaaning Jensen, Bo¹  Fishburn, C.Simone¹ 

[1] Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Murine D2 dopamine receptor (D2;    D3;    D4);    Bumetanide;    Dopamine;    Xenopus oocyte;    Na+/K+/2Cl− cotransporter;    BSA;    bovine serum albumin;    CNS;    central nervous system;    DAG;    diacylglycerol;    G protein;    GTP-binding protein;    HEPES;    4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid;    Ins-1;    4;    5-P3;    inositol-1;    4;    5-trisphosphate;    PKC;    protein kinase C;    PKA;    protein kinase A;    PLC;    phospholipase C;    PTX;    pertussis toxin;   

DOI  :  10.1016/S0014-5793(97)01505-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The different murine D2-type dopamine receptors (D_2L, D_2S, D_3L, D_3S, and D₄) were expressed in Xenopus laevis oocytes. The D2-type receptors were all similarly and efficiently expressed in Xenopus oocytes and were shown to bind the D2 antagonist [¹²⁵I]sulpride. They were all shown to activate Cl⁻ influx upon agonist stimulation. Using the diagnostic inhibitor bumetanide, we were able to separate the Na⁺/K⁺/2Cl⁻ cotransporter component of the Cl⁻ influx from the total unidirectional Cl⁻ influx. The D_3L subtype was found to operate exclusively through the bumetanide-insensitive Cl⁻ influx whereas the other D2-type receptors acted on the Na⁺/K⁺/2Cl⁻ cotransporter as well. The pertussis toxin sensitivity of the receptor-activated chloride influx via the Na⁺/K⁺/2Cl⁻ cotransporter varied between the various D2-type receptors showing that they may couple to different G proteins, and activate different second messenger systems.

【 授权许可】

Unknown