Human D3 dopamine receptor in the medulloblastoma TE671 cell line: cross‐talk between D1 and D3 receptors

FEBS Letters

Levavi-Sivan, Berta¹  Park, Bae-Hang¹  Fuchs, Sara¹  Fishburn, C.Simone¹ 

[1] Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Dopamine receptor;    D1;    D3;    TE671;    Human;   

DOI  :  10.1016/S0014-5793(98)01356-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In search of a cell line in which the D₃ dopamine receptor is expressed endogenously, we found that the neuron-derived human medulloblastoma cell line TE671 expresses the human D₃ (hD₃) and D₁ (hD₁) receptor, but neither the D₂ or D₄ receptors. Exposure of TE671 cells to the D₃ agonist 7-OH-DPAT (DPAT), or to the D₁ agonist SKF-38393 (SKF) increased the expression of hD₃ or hD₁ mRNA, respectively. Moreover, whereas DPAT had no effect on hD₁ mRNA levels, stimulating the cells with SKF caused an increase in both hD₁ and hD₃ transcript levels. These results suggest (i) that following ligand stimulation, hD₃ and hD₁ receptors are upregulated to enhance their own receptor expression, and (ii) that upregulation of hD₁ receptor transcripts leads to a stimulation of the hD₃ dopamine receptor transcripts.

【 授权许可】

Unknown