Modifications of proteoglycans extracted from monolayer cultures of young and senescent human skin fibroblasts

FEBS Letters

Campagnari, Francesco¹  Albertini, Riccardo¹  Passi, Alberto¹  De Luca, Giancarlo¹ 

[1] Department of Biochemistry `A. Castellani', II Faculty of Medicine and Surgery, University of Pavia, via Taramelli 3b, 27100 Pavia, Italy

关键词: Proteoglycan;    Glycosaminoglycan;    Fibroblast;    Culture monolayer;    Replicative senescence;   

DOI  :  10.1016/S0014-5793(97)01515-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteoglycans (PGs) were extracted from culture monolayers of human skin fibroblasts (HFs) at early and late passages. Total PGs from senescent cells had markedly reduced abilities to bind type I collagen and hyaluronic acid, but retained normal binding properties with fibronectin and laminin. The constituent polysaccharides of PGs were comparatively characterised. PGs recovered from young and senescent HF cultures had equivalent total polyanionic charges and similar size distributions of the glycosaminoglycan chains. This applied to both types of polysaccharide chains found in PGs, namely the galactosaminoglycuronans (GalN-GAGs) and the glucosaminoglycuronans (GlcN-GAGs). However, senescent HFs produced a greater proportion of PGs containing GlcN-GAG chains and increased the sulphation of the remainding PG fraction with GalN-GAG moieties, yielding a major gain of C6-sulphate groups in the galactosamine residues.

【 授权许可】

Unknown