Osteopontin involvement in integrin‐mediated cell signaling and regulation of expression of alkaline phosphatase during early differentiation of UMR cells

FEBS Letters

Fujii, Noriko²  Nemoto, Atsuko¹  Liu, Yin-Kun¹  Tateishi, Tetsuya¹  Uemura, Toshimasa¹  Yabe, Tomoko¹  Ushida, Takashi¹ 

[1] Bionic Design Group, National Institute for Advanced Interdisciplinary Research (NAIR), Tsukuba Research Center, Ibaraki 305, Japan;Field and Reaction, PRESTO, JST, National Institute of Bioscience and Human Technology (NIBH), Tsukuba Research Center, Ibaraki 305, Japan

关键词: Osteopontin;    Focal adhesion kinase;    Osteoblast;    Alkaline phosphatase;    SDS;    sodium dodecyl sulfate;    PMSF;    phenylmethylsulfonyl fluoride;    PBS;    phosphate buffered saline;    BSA;    bovine serum albumin;    α-MEM;    α-minimal essential medium;   

DOI  :  10.1016/S0014-5793(97)01498-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the function of osteopontin in osteoblast differentiation, we have examined the signal transduction pathway in an osteoblastic cell line (UMR106-6) bound to osteopontin, fibronectin, vitronectin and collagen type I surfaces. This was done by investigating the production and autophosphorylation of focal adhesion kinase (FAK) and the expression of alkaline phosphatase (ALP) at the transcription level. Results suggest that osteopontin was not only responsible for the autophosphorylation of FAK but regulated the expression of ALP, which was strongly correlated with FAK activity. These results suggest that osteopontin might act as a trigger in the early differentiation of osteoblasts.

【 授权许可】

Unknown