Sequence and characterisation of the RET proto‐oncogene 5′ flanking region: analysis of retinoic acid responsiveness at the transcriptional level

FEBS Letters

Puliti, Aldamaria¹  Bocciardi, Renata¹  Patrone, Giovanna¹  Ravazzolo, Roberto²  Romeo, Giovanni¹ 

[1] Università di Genova, Facoltà di Medicina e Laboratorio di Genetica Molecolare, Istituto G. Gaslini, Largo G. Gaslini, 5, 16148 Genoa, Italy;Istituto di Biologia e Genetica, Università di Genova, V.le Benedetto XV 6, 16132 Genoa, Italy

关键词: RET proto-oncogene;    Retinoic acid response element;    Neuroblastoma;    Hirschsprung;    Multiple endocrine neoplasia type 2;    Medullary thyroid carcinoma;   

DOI  :  10.1016/S0014-5793(97)01435-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up-regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5′ to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.

【 授权许可】

Unknown