Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1

FEBS Letters

Sakurai, Hidehiro¹  Mochimaru, Mari¹ 

[1] Department of Biology, School of Education, Waseda University, Nishiwaseda 1, Shinjuku, Tokyo 169-50, Japan

关键词: Chloroplast ATP synthase;    Chloroplast coupling factor 1;    F1-ATPase;    Energy-transfer inhibitor;    Tentoxin;    CF1;    chloroplast coupling factor 1;    C·T n;    CF1 which binds n molecules of tentoxin;    K d;    dissociation constant;    PCA;    perchloric acid;   

DOI  :  10.1016/S0014-5793(97)01421-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tentoxin binding on chloroplast coupling factor 1 (CF₁) was studied using a centrifugation column method followed by HPLC analysis. From non-linear regression analysis of the results, the presence of three types of binding site with the following K _d values was deduced: 6.9×10⁻⁸ M (first site), 1.4×10⁻⁵ M (second site), and 6.3×10⁻³ M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF₁. The forward rate constant of the binding of tentoxin on the first site was 6.3×10³ M⁻¹ s⁻¹.

【 授权许可】

Unknown