FEBS Letters | |
Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1 | |
Sakurai, Hidehiro1  Mochimaru, Mari1  | |
[1] Department of Biology, School of Education, Waseda University, Nishiwaseda 1, Shinjuku, Tokyo 169-50, Japan | |
关键词: Chloroplast ATP synthase; Chloroplast coupling factor 1; F1-ATPase; Energy-transfer inhibitor; Tentoxin; CF1; chloroplast coupling factor 1; C·T n; CF1 which binds n molecules of tentoxin; K d; dissociation constant; PCA; perchloric acid; | |
DOI : 10.1016/S0014-5793(97)01421-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Tentoxin binding on chloroplast coupling factor 1 (CF1) was studied using a centrifugation column method followed by HPLC analysis. From non-linear regression analysis of the results, the presence of three types of binding site with the following K d values was deduced: 6.9×10−8 M (first site), 1.4×10−5 M (second site), and 6.3×10−3 M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF1. The forward rate constant of the binding of tentoxin on the first site was 6.3×103 M−1 s−1.
【 授权许可】
Unknown
【 预 览 】
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