The partial purification of a factor from Dunaliella salina that causes the rapid in situ inactivation of light‐activated chloroplast coupling factor 1 (CF1)

FEBS Letters

Selman, Bruce R.¹  Selman-Reimer, Susanne¹ 

[1] Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA

关键词: Chloroplast coupling factor 1;    ATPase;    (Dunaliella salina);    CF1;    chloroplast coupling factor 1;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(88)80633-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A factor having the expected properties of the in vivo oxidant responsible for inactivating the in vivo light-activated chloroplast coupling factor 1 (CF₁) has been partially purified from cell-free extracts of Dunaliella salina. This factor is highly polar, weakly acidic, and relatively temperature stable. The ability of this factor to inactivate light-activated CF₁ is prevented if it is pretreated with reductants such as dithiothreitol. The factor has virtually no effect on the ethanol-induced, Mg²⁺ -dependent ATPase activity of the isolated CF₁.

【 授权许可】

Unknown