A sulfatide receptor distinct from L‐selectin is involved in lymphocyte activation

FEBS Letters

Suzuki, Takashi¹  Suzuki, Yasuo¹  Kawashima, Hiroto³  Ward, Peter A²  Ding, Ziqiang³  Miyasaka, Masayuki³ 

[1] Department of Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka 422, Japan;Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-0602, USA;Department of Bioregulation, Biomedical Research Center, Osaka University Medical School, Suita 565, Japan

关键词: L-Selectin;    Glycolipid;    Cell adhesion molecule;    Phorbol 12-myristate 13-acetate;    Cell aggregation;    Lymphocyte;   

DOI  :  10.1016/S0014-5793(97)01400-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sulfatide induces leukocyte activation, which is thought to be mediated via L-selectin. Here we demonstrate that lymphocytes express a receptor for sulfatide distinct from L-selectin and that this receptor is involved in sulfatide-induced cell activation. While cell surface L-selectin expression was abolished by phorbol 12-myristate 13-acetate (PMA), lymphocytes retained the ability to bind sulfatide in liquid phase as well as in immobilized solid phase. The novel sulfatide receptor obtained from PMA-treated lymphocytes showed a molecular size of 65 kDa. Stimulation through this receptor triggered cytosolic free Ca²⁺ elevation and intercellular aggregation.

【 授权许可】

Unknown