Lactoferrin inhibits the binding of lipopolysaccharides to L‐selectin and subsequent production of reactive oxygen species by neutrophils

FEBS Letters

Baveye, Sophie¹  Mazurier, Joël¹  Elass, Elisabeth¹  Legrand, Dominique¹ 

[1] Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS no. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France

关键词: Lactoferrin;    L-Selectin;    Lipopolysaccharide;    Neutrophil;    Oxygen free radical;    Lf;    lactoferrin;    hLf;    human lactoferrin;    LPS;    lipopolysaccharides;    LBP;    LPS-binding protein;   

DOI  :  10.1016/S0014-5793(00)01243-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activation of leukocytes by lipopolysaccharides (LPS), resulting in the oxidative burst, contributes to the pathogenesis of septic shock. The binding of LPS to L-selectin, which was reported as a serum-independent LPS receptor on neutrophils, induces the production of oxygen free radicals. Human lactoferrin (hLf), an anti-inflammatory glycoprotein released from neutrophil granules during infection, binds to LPS. In this study, we investigated the capacity of hLf to inhibit the L-selectin-mediated activation of neutrophils. Our experiments revealed that hLf prevents the binding of LPS to L-selectin in a concentration-dependent manner. Inhibition was maximum (87.7±0.5%) at a concentration of 50 μg/ml of hLf. Furthermore, hLf inhibited up to 55.4±0.5% of the intracellular hydrogen peroxide production induced by LPS in neutrophils. These findings suggest that the anti-inflammatory properties of hLf are due, at least in part, to their ability to prevent the binding of LPS to neutrophil L-selectin.

【 授权许可】

Unknown