Mitochondrial respiratory chain deficiency leads to overexpression of antioxidant enzymes

FEBS Letters

Cairo, Gaetano¹  Brambilla, Liliana²  Azzi, Angelo³  Cantoni, Orazio²  Sestili, Piero²  O'Donnel, Valery³ 

[1] Centro di Studio sulla Patologia Cellulare, Consiglio Nazionale delle Ricerche and Istituto di Patologia Generale, Università di Milano, Milan, Italy;Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara 27, 61029 Urbino, Italy;Institut für Biochemie und Molekularbiologie der Universität Bern, Bern, Switzerland

关键词: Respiratory deficiency;    Antioxidant enzyme;    Glutathione peroxidase;    Heme oxygenase;    Oxidative stress;    GPx;    glutathione peroxidase;    EtBr;    ethidium bromide;    CP;    chloramphenicol;    HO-1;    heme oxygenase;   

DOI  :  10.1016/S0014-5793(97)01393-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

U937 cell growth in the presence of either chloramphenicol or ethidium bromide rapidly leads to respiratory deficiency. The novel finding of this report is that this response is paralleled by a specific increase in Se-dependent and -independent glutathione peroxidase activities as well as of glutathione peroxidase and heme oxygenase mRNAs. Under the same experimental conditions, catalase activity and catalase mRNA do not show appreciable changes. These results can be explained by an increased formation of H₂O₂ at the early times of development of respiratory deficiency followed by induction of antioxidant enzymes.

【 授权许可】

Unknown