Plant CDC2 is not only targeted to the pre‐prophase band, but also co‐localizes with the spindle, phragmoplast, and chromosomes

FEBS Letters

Inzé, Dirk¹  Traas, Jan²  Engler, Gilbert¹  Stals, Hilde¹  Bauwens, Serge¹  Van Montagu, Marc¹ 

[1]Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

[2]Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026 Versailles Cedex, France

关键词: Arabidopsis thaliana;    Cell cycle;    Immunolocalization;    Medicago sativa;    Mitosis;    p34cdc2 kinase;    BSA;    bovine serum albumin;    CDC;    cell division cycle;    CDK;    cyclin-dependent kinase;    CLSM;    confocal laser scanning microscopy;    DAPI;    4′;    6-diamidino-2-phenylindole;    EGTA;    ethylene glycol-bis(β-aminoethyl ether) N;    N;    N′;    N′-tetraacetic acid;    MAP;    microtubule-associated protein;    MTSB;    microtubule-stabilizing buffer;    PIPES;    piperazine-N;    N′-bis(ethanesulfonic acid);    PPB;    pre-prophase band;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    STBI;    soybean trypsin inhibitor;    Tween;    polyoxyethylenesorbitan;   

DOI  :  10.1016/S0014-5793(97)01368-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A polyclonal antiserum against the p34^cdc2 homologue of Arabidopsis thaliana, CDC2aAt, was used in parallel with a polyclonal antiserum against the PSTAIRE motif to study the subcellular localization of CDC2 during the cell cycle of isolated root tip cells of Medicago sativa. During interphase, CDC2 was located in the nucleus and in the cytoplasm. The cytoplasmic localization persisted during the complete cell cycle, whereas the nuclear signal disappeared at nuclear envelope breakdown. At the beginning of anaphase, the anti-CDC2aAt antibody transiently co-localized with condensed chromosomes. The chromosomal co-localization disappeared as anaphase continued and remained excluded from the separated chromosomes until cytokinesis, when CDC2 re-located to the newly forming nuclei. We also observed a co-localization of CDC2 with three microtubular structures, the pre-prophase band, the spindle, and the phragmoplast.

【 授权许可】

Unknown