Dissecting the assembly pathway of the 20S proteasome

FEBS Letters

Baumeister, Wolfgang²  Seemüller, Erika²  Golbik, Ralph¹  Zühl, Frank² 

[1] Martin Luther University Halle-Wittenberg, D-06120 Halle, Germany;Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany

关键词: Proteasome;    Rhodococcus;    Processing;    Assembly;    Propeptide;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    AMC;    7-amino-4-methylcoumarin;    Suc;    succinyl;    pre;    precursor;    pro;    propeptide;    βΔ;    β-subunit with propeptide deleted;   

DOI  :  10.1016/S0014-5793(97)01370-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteasomes reach their mature active state via a complex cascade of folding, assembly and processing events. The Rhodococcus proteasome offers a means to dissect the assembly pathway and to characterize intermediates; its four subunits (α₁, α₂, β₁, β₂) assemble efficiently in vitro with any combination of α and β. Assembly studies with wild-type and N-terminally truncated β-subunits in conjunction with refolding studies allowed to define the role of the propeptide which is two-fold: It supports the initial folding of the β-subunits and it promotes the maturation of the holoproteasomes.

【 授权许可】

Unknown