GC rich DNA oligonucleotides with narrow minor groove width

FEBS Letters

Dai, Linsen¹  Umemoto, Kimiko¹  Sarma, Mukti H.¹  Sarma, Ramaswamy H.¹ 

[1] Institute of Biomolecular Stereodynamics, Chemistry, State University of New York at Albany, Albany, NY 12222, USA

关键词: GC rich DNA oligomer;    Minor groove width;    2D NMR;    2D NOESY in water;    Pyrimidine-purine junction;    Purine-pyrimidine junction;   

DOI  :  10.1016/S0014-5793(97)01351-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Investigation of the width of the minor groove using 500 MHz NMR spectroscopy in three closely related 11-mer B-DNA duplexes shows that the minor groove is narrow in a GC rich oligonucleotide, and that a narrow minor groove is not something endemic to DNAs with persistent repetitions of adenine nucleotides (A-tract DNA). The width of the groove is dictated by local sequence contexts and independent of neighboring A-tract DNA.

【 授权许可】

Unknown