Solution structure of d‐GAATTCGAATTC by 2D NMR

FEBS Letters

Miles, H.Todd¹  Hosur, R.V.¹  Sheth, Anu¹  Ravikumar, M.¹  Zu-Kun, Tan¹  Govil, Girjesh¹  Chary, K.V.R.¹ 

[1] Chemical Physics Group, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400005, India

关键词: 2D NMR;    Oligonucleotides solution structure;    Sugar geometry;   

DOI  :  10.1016/0014-5793(86)80868-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new approach based on the correlated spectroscopy (COSY) in 2D NMR has been described for determination of sugar geometries in oligonucleotides. Under the usual low resolution conditions employed in COSY, the intensities of cross peaks depend on the magnitudes of coupling constants. There are five vicinal coupling constants in a deoxyribose ring which are sensitive to the sugar geometry. The presence, absence and rough comparison of relative intensities of COSY cross peaks arising from such coupling constants enable one to fix the sugar conformation to a fair degree of precision. The methodology has been applied to d-GAATTCGAATTC. It is observed that ten out of the twelve nucleotide units in this sequence exhibit a rare Ol′-endo geometry. The EcoRI cleavage sites (between G and A) in the dodecanucleotide show an interesting variation in the conformation with the two sugars attached to the Gs acquiring a geometry between C2′-endo and C4′-endo.

【 授权许可】

Unknown