Cytotoxicity of spin trapping compounds

FEBS Letters

Baeger, Ingo¹  Grigor'ev, Igor A²  Rohde, Elvira¹  Haseloff, Reiner F¹  Blasig, Ingolf E¹  Mertsch, Katharina¹ 

[1] Research Institute of Molecular Pharmacology, Alfred-Kowalke-Str. 4, 10315 Berlin, Germany;Institute of Organic Chemistry, Novosibirsk, Russia

关键词: Cytotoxicity;    Spin trap;    Endothelial cell;    ACE;    angiotensin converting enzyme;    BAEC;    bovine aortic endothelial cells;    PBN;    N-tert-butyl-α-phenyl nitrone;    CMIO;    4-carboxy-2;    2-dimethyl-2H-imidazole-1-oxide;    CYIO;    4-cyano-2;    2-dimethyl-2H-imidazole-1-oxide;    DBNBS;    3;    5-dibromo-4-nitroso-benzenesulfonic acid;    NN 2;    4;    4-dimethyl-2;    5-diphenyl-5-methoxy-2-imidazoline-3-oxide-1-oxyl;    MCIO;    2;    2-dimethyl-4-methoxycarbonyl-2H-imidazole-1-oxide;    DMPO;    5;    5-dimethylpyrroline-1-oxide;    NN 3;    4;    4-dimethyl-2;    5;    5-triphenyl-2-imidazoline-3-oxide-1-oxyl;    MNP;    2-methyl-2-nitrosopropane;    NR;    neutral red;    NB;    nitrosobenzene;    POBN;    α-(4-pyridyl-1-oxide)-N-tert-butyl nitrone;    TEMPO;    2;    2;    6;    6-tetramethylpiperidine-1-oxyl;    TMPO;    3;    3;    5;    5-tetramethylpyrroline-1-oxide;    NN 1;    4;    4;    5;    5-tetramethyl-2-(4-trimethylammoniophenyl)-2-imidazoline-3-oxide-1-oxyl methyl sulfate;    TMIO;    2;    2;    4-trimethyl-2H-imidazole-1-oxide;   

DOI  :  10.1016/S0014-5793(97)01349-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spin trapping compounds are used frequently to detect free radicals released by cells. Their cytotoxicity has to be considered in order to prevent perturbations of normal cell growth and viability. Eleven spin traps (eight nitrones and three nitroso traps) have been tested for their effects on bovine aortic endothelial cells (toxicity range, 50% survival rate). The lowest cytotoxicity was found for 5,5-dimethylpyrroline-1-oxide and 2,2,4-trimethyl-2H-imidazole-1-oxide whereas nitrosobenzene and 2-methyl-2-nitrosopropane exerted the strongest cytotoxic effects. In addition, three nitronyl nitroxides were tested. Their cytotoxicity was found to be dependent on substitution, and the toxic concentration of a lipophilic derivative was found to be more than two orders lower as compared to a hydrophilic derivative. The results of this study indicate that most spin traps can be used in cell cultures at customary (i.e. millimolar) concentrations; caution is recommended when nitroso spin traps are applied to cells.

【 授权许可】

Unknown