Tricyclic antidepressants block cholinergic nicotinic receptors and ATP secretion in bovine chromaffin cells

FEBS Letters

González-Garcı́a, Carmen¹  Izaguirre, Victor¹  Fernández-Fernández, José M¹  Ceña, Valentı́n¹ 

[1]Departamento de Farmacologı́a and Instituto de Neurociencias, Universidad Miguel Hernández, Campus de San Juan, E-03550 San Juan (Alicante), Spain

关键词: Nicotine;    ATP secretion;    Chromaffin cell;    Imipramine;    Desipramine;    Calcium channel;   

DOI  :  10.1016/S0014-5793(97)01343-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nicotine-induced ATP secretion from chromaffin cells was blocked by imipramine and desipramine. This blocking action took place on both, fast and slow, components of ATP secretion. Exposure of chromaffin cells to nicotine (10 μM) for 4 s induced an inward current of about −155 pA. Imipramine and desipramine blocked, in a concentration-dependent manner, both peak inward current and total charge influx in response to nicotine. In addition, imipramine and desipramine partially (40%) blocked depolarization-induced ATP secretion and Ca²⁺ currents evoked by high K⁺. This suggests that tricyclic antidepressants block nicotine-induced ATP secretion by acting on two targets: one is the nicotinic receptor itself and the second one are voltage-dependent Ca²⁺ channels.

【 授权许可】

Unknown