Nuclear translocation of the Y‐box binding protein by ultraviolet irradiation

FEBS Letters

Kuwano, Michihiko²  Ohga, Takefumi²  Uchiumi, Takeshi²  Wada, Morimasa²  Kohno, Kimitoshi¹  Toh, Satoshi²  Koike, Koji² 

[1] Department of Molecular Biology, University of Occupational and Environmental Health, Kitakyushu 807, Japan;Department of Biochemistry, Kyushu University School of Medicine, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-82, Japan

关键词: YB-1;    UV irradiation;    Nuclear translocation;    Protein kinase C;    Green fluorescent protein;   

DOI  :  10.1016/S0014-5793(97)01296-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Y-box binding protein, YB-1, is a member of a DNA binding protein family with a structurally and functionally conserved cold shock domain. Using Western blotting and immunohistochemical methods, larger amounts of YB-1 were detected in the cytosol, particularly at the perinuclear region, than in the nucleus of human cancer cells. UV irradiation increased accumulation of YB-1 in the nucleus at 20 min and thereafter. This translocation of YB-1 into the nucleus by UV irradiation was blocked by the protein kinase inhibitor H-7, but not HA-1004. Both green fluorescent protein (GFP)-YB-1 and GFP-YB-1C with the C-terminus (248–317) of YB-1 were located mainly in the cytosol, but GFP-YB-1ΔC with a deletion at the C-terminus of YB-1 was located in the nucleus. YB-1 is translocated into the nucleus by UV irradiation, possibly through a protein kinase C-mediated signal transduction pathway, and the C-terminal region of YB-1 might be important for cytoplasmic retention of YB-1.

【 授权许可】

Unknown