Nitric oxide mediates NMDA‐evoked [3H]GABA release from chick retina cells

FEBS Letters

Macaione, Vincenzo¹  Picciurro, Vincenzo¹  Macaione, Salvatore¹  Pedale, Santa¹  Fabiano, Caterina¹  Ientile, Riccardo¹ 

[1] Institute of Biochemical Sciences and Clinical Biochemistry, Faculty of Medicine, University of Messina, viale Italia, 137, 98122 Messina, Italy

关键词: Nitric oxide;    Chick embryo;    Retina cell;    NMDA;    GABA release;    NO;    nitric oxide;    8-Br-cGMP;    8-bromoguanosine 3′:5′-cyclic monophosphate;    BH4;    tetra-hydrobiopterin;    MK-801;    (+)5-methyl-10;    11-dihydro-5H-dibenzo-[a;    d]cyclohepten-5;    10-imine hydrogen maleate;    LY 274614;    ±3R*;    4aS;    8aR*-6-(phosphomethyl)decahydro-isoquinoline-3-carboxylic;    L-NA;    N ω-nitro-l-arginine;    D-NA;    N ω-nitro-d-arginine;    NMDA;    N-methyl-d-aspartate;    IBMX;    isobutyl-methylxanthine;    Hb;    Haemoglobin;   

DOI  :  10.1016/S0014-5793(97)01322-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The stimulation of NMDA receptor increased [³H]GABA release from preloaded cultured retina cells. This effect appears to be mediated by NO production, since addition of L-NA reduces NMDA-evoked [³H]GABA release. Spermine/NO complex, an NO donor, mimics the effect produced by NMDA. The addition of zaprinast, a phosphodiesterase inhibitor, as well as 8-Br-cGMP enhances the NMDA-evoked [³H]GABA release. These results agree with the existence in chick retina cells of NO/cGMP pathways and support a role for NO in NMDA-evoked events. The activation of this receptor complex through maturative stages of the retina together with the NO-mediated increase in GABA release may account for NMDA differentiative effect in culturing retina cells.

【 授权许可】

Unknown