FEBS Letters | |
Nitric oxide mediates NMDA‐evoked [3H]GABA release from chick retina cells | |
Macaione, Vincenzo1  Picciurro, Vincenzo1  Macaione, Salvatore1  Pedale, Santa1  Fabiano, Caterina1  Ientile, Riccardo1  | |
[1] Institute of Biochemical Sciences and Clinical Biochemistry, Faculty of Medicine, University of Messina, viale Italia, 137, 98122 Messina, Italy | |
关键词: Nitric oxide; Chick embryo; Retina cell; NMDA; GABA release; NO; nitric oxide; 8-Br-cGMP; 8-bromoguanosine 3′:5′-cyclic monophosphate; BH4; tetra-hydrobiopterin; MK-801; (+)5-methyl-10; 11-dihydro-5H-dibenzo-[a; d]cyclohepten-5; 10-imine hydrogen maleate; LY 274614; ±3R*; 4aS; 8aR*-6-(phosphomethyl)decahydro-isoquinoline-3-carboxylic; L-NA; N ω-nitro-l-arginine; D-NA; N ω-nitro-d-arginine; NMDA; N-methyl-d-aspartate; IBMX; isobutyl-methylxanthine; Hb; Haemoglobin; | |
DOI : 10.1016/S0014-5793(97)01322-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The stimulation of NMDA receptor increased [3H]GABA release from preloaded cultured retina cells. This effect appears to be mediated by NO production, since addition of L-NA reduces NMDA-evoked [3H]GABA release. Spermine/NO complex, an NO donor, mimics the effect produced by NMDA. The addition of zaprinast, a phosphodiesterase inhibitor, as well as 8-Br-cGMP enhances the NMDA-evoked [3H]GABA release. These results agree with the existence in chick retina cells of NO/cGMP pathways and support a role for NO in NMDA-evoked events. The activation of this receptor complex through maturative stages of the retina together with the NO-mediated increase in GABA release may account for NMDA differentiative effect in culturing retina cells.
【 授权许可】
Unknown
【 预 览 】
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