Purified HrpA of Pseudomonas syringae pv. tomato DC3000 reassembles into pili

FEBS Letters

Roine, Elina¹  Romantschuk, Martin¹  Saarinen, Juhani²  Kalkkinen, Nisse² 

[1] Department of Biosciences, Division of General Microbiology, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland;Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland

关键词: Type III secretion;    HrpA;    Hrp pili;    Plant-microbe interaction;    Pseudomonas syringae;   

DOI  :  10.1016/S0014-5793(97)01276-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pseudomonas syringae pv. tomato DC3000 produces Hrp pili under inducing in vitro conditions. A preparation of partially purified extracellular filaments contains HrpA, flagellin and some minor contaminants. HrpA was separated from the major contaminant, the flagellin, by gel filtration to a fraction containing HrpA as well as its three N-terminally truncated forms. These were further separated by two steps of reversed phase chromatography. HrpA and its degradation products were each shown to reassemble into filament structures after denaturation and renaturation showing that HrpA alone is sufficient for formation of filament structures.

【 授权许可】

Unknown