Functional assessment of the yeast Rvs161 and Rvs167 protein domains

FEBS Letters

Aigle, Michel¹  Sivadon, Pierre¹  Crouzet, Marc² 

[1] Laboratoire de Biologie Cellulaire de la Levure, IBGC UPR CNRS 9026, 1 rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France;Laboratoire de Biologie Moléculaire et de Séquençage, UPR CNRS 9026, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France

关键词: RVS167;    RVS161;    SH3 domain;    Actin cytoskeleton;    Budding pattern;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(97)01248-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutations in RVS161 and RVS167 yeast genes induce identical phenotypes associated to actin cytoskeleton disorders. The whole Rvs161 protein is similar to the amino-terminal part of Rvs167p, thus defining a RVS domain. In addition to this domain, Rvs167p contains a central glycine-proline-alanine rich domain and a SH3 domain. To assess the function of these different domains we have expressed recombinant Rvs proteins in rvs mutant strains. Phenotype analysis has shown that the RVS and SH3 domains are necessary for phenotypical complementation, whereas the GPA domain is not. Moreover, we have demonstrated that the RVS domains from Rvs161p and Rvs167p have distinct roles, and that the SH3 domain needs the specific RVS domain of Rvs167p to function. These results suggest that Rvs161p and Rvs167p play distinct roles, while acting together in a common function.

【 授权许可】

Unknown