FEBS Letters | |
The SH3 domain of the tight junction protein ZO‐1 binds to a serine protein kinase that phosphorylates a region C‐terminal to this domain | |
Anderson, James M.1  Balda, Maria S.2  Matter, Karl2  | |
[1] Department of Internal Medicine and Cell Biology, Yale School of Medicine, New Haven, CT, USA;Department of Cell Biology, Science III, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland | |
关键词: Tight junction; ZO-1; SH3 domain; Serine protein kinase; ZO-1; zonula occludens-1; ZO-2; zonula occludens-2; SH3; Src homology domain 3; GST; glutathione S-transferase; PDZ domain; PSD95-Dlg A-ZO-1 homology domain; ZAK; ZO-1 Associated Kinase; | |
DOI : 10.1016/S0014-5793(96)01352-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
ZO-1 is a tight junction phosphoprotein partially homologous to a tumor suppressor in Drosophila. The homologous region contains an SH3 domain with an unidentified function. Using fusion proteins containing the SH3 domain and various N- and C-terminal sequences, we tested for association of a kinase with this protein domain in extracts of MDCK cells. We show that the SH3 domain of ZO-1 binds a serine protein kinase that phosphorylates a region immediately C-terminal to the SH3 domain. This kinase associates specifically with the SH3 domain of ZO-1 and appears to be also associated with junctional complexes extracted from MDCK cells.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020303721ZK.pdf | 800KB | download |