FEBS Letters | |
Is the mammalian porin channel, VDAC, a perfect cylinder in the high conductance state? | |
Krasilnikov, O.V1  Yuldasheva, L.N2  Nogueira, R.A2  Campos de Carvalho, A.C3  Carneiro, C.M.M2  | |
[1] Laboratory of Molecular Physiology, Institute of Physiology and Biophysics, 700095 Tashkent, Uzbekistan;Laboratory of Membrane Biophysics, Center of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Av. prof. Moraes Rego, s/n, Cidade Universitaria, 50670-901 Recife, PE, Brazil;Institute of Biophysics Carlos Chagas Filho, UFRJ, Rio de Janeiro, RJ, Brazil | |
关键词: Voltage-dependent anion-selective channel; Ion channel; Size; | |
DOI : 10.1016/S0014-5793(97)01198-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The mammalian porin channel (VDAC, porin-31BM) was reconstituted in planar lipid bilayers under voltage clamp conditions. The radii of both entrances of the channel were examined using a method that consisted in filling the channel with different non-electrolytes through its cis or trans entrances while recording single channel conductances. As a result it was found that the geometry of channels formed by porin-31BM could not be approximated by a perfectly cylindrical pore. In fact there is an asymmetry in the geometry of the channel: the diameters of the cis and trans entrances were estimated to be ∼2 nm and ∼4 nm respectively.
【 授权许可】
Unknown
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