Inhibition of DNA synthesis and tube morphogenesis of cultured vascular endothelial cells by chondromodulin‐I

FEBS Letters

Shukunami, Chisa²  Sato, Mutsumi¹  Hiraki, Yuji²  Kono, Takaharu¹  Kondo, Jun¹ 

[1] Research Center, Mitsubishi Chemical Corporation, Kanagawa 227, Japan;Department of Biochemistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565, Japan

关键词: ChM-I;    Vascular endothelial cell;    Tube morphogenesis;    Growth inhibitor;   

DOI  :  10.1016/S0014-5793(97)01151-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cartilage is an avascular tissue, and exhibits anti-angiogenic properties. Cartilage extracts have been shown to contain an inhibitor for DNA synthesis in vascular endothelial cells in vitro. Here we purified the inhibitory activity in the 10–50 kDa fraction of guanidine extracts from fetal bovine epiphyseal cartilage, and found that the inhibitor was identical with chondromodulin-I (ChM-I). Purified ChM-I inhibited tube morphogenesis of cultured vascular endothelial cells, as well as DNA synthesis. These results indicate that cartilage-specific glycoprotein ChM-I may participate in the maintenance of avascularity and anti-angiogenic properties of cartilage.

【 授权许可】

Unknown