Cross‐linked trimers of bovine ribonuclease A: activity on double‐stranded RNA and antitumor action

FEBS Letters

Gotte, Giovanni¹  Libonati, Massimo¹  Costanzo, Chiara¹  Sorrentino, Salvatore³  Armato, Ubaldo²  Testolin, Lucia² 

[1] Institute of Biological Chemistry, Faculty of Medicine, The University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy;Institute of Anatomy and Histology, Faculty of Medicine, The University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy;Department of Organic and Biological Chemistry, The University of Naples, Via Mezzocannone 16, I-80134 Naples, Italy

关键词: RNase A;    RNase A oligomer;    Double-stranded RNA;    Tumor;    Antitumor action;   

DOI  :  10.1016/S0014-5793(97)01147-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trimers of bovine pancreatic RNase A were obtained by cross-linking native RNase A with dimethyl suberimidate. They degrade double-stranded RNA more efficiently than dimers and monomers of RNase A, and display significant cytotoxic and/or cytostatic actions against C4-I cells (a human cell line derived from squamous carcinoma of the uterus cervix). On the same cell line cross-linked dimers of RNase A appear to be ineffective.

【 授权许可】

Unknown