Identification of positively charged residues contributing to the stability of plasminogen activator inhibitor 1

FEBS Letters

Declerck, Paul J²  Gils, Ann²  Aertgeerts, Kathleen¹  Lu, Jie²  Knockaert, Isabelle² 

[1]Laboratory for Analytical Chemistry and Medicinal Physicochemistry, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

[2]Laboratory for Pharmaceutical Biology and Phytopharmacology, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium

关键词: Plasminogen activator inhibitor 1;    Serpin;    Functional stability;    Mutagenesis;    PAI-1;    plasminogen activator inhibitor 1;    PAI-1-R186E;    R187E;    PAI-1 in which Arg186 and Arg187 have been replaced by Glu;    PAI-1-H190E;    K191E;    PAI-1 in which His190 and Lys191 have been replaced by Glu;    PAI-1-H190L;    K191L;    PAI-1 in which His190 and Lys191 have been replaced by Leu;    wt;    wild-type;    serpin;    serine proteinase inhibitor;    t-PA;    tissue-type plasminogen activator;    u-PA;    urokinase-type plasminogen activator;    PBS;    phosphate buffered saline;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(97)01122-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasminogen activator inhibitor 1 (PAI-1), a member of the serpins, has a unique conformational flexibility. A typical characteristic is its intrinsic lability resulting in the conversion of the active conformation to a latent conformation. In the present study, we have evaluated the effect of substitution of positively charged residues located at the turn connecting strand s4C with strand s3C, either with negatively charged or with neutral residues, on the functional stability of PAI-1. The following mutants were constructed, purified and characterized in comparison to wild-type (wt) PAI-1: PAI-1-R186E,R187E (Arg¹⁸⁶→Glu and Arg¹⁸⁷→Glu), PAI-1-H190E,K191E (His¹⁹⁰→Glu and Lys¹⁹¹→Glu) and PAI-1-H190L,K191L (His¹⁹⁰→Leu and Lys¹⁹¹→Leu). In contrast to wtPAI-1 the mutants exhibited no inhibitory activity. Whereas latent wtPAI-1 can be reactivated (up to a specific activity of 78±19%) by treatment with guanidinium chloride, a similar treatment applied to these mutants resulted in a significant but relatively small increase of specific activity (i.e. to 14%). Evaluation of the functional stability (at 37°C, pH 5.5, 1 M NaCl) revealed a strongly decreased functional stability compared to wtPAI-1 (i.e. 3–9 h for the mutants vs. >24 h for wtPAI-1). Further characterization by heat denaturation studies and plasmin susceptibility confirmed that removal or reversal of the positive charge on the turn connecting s4C with s3C results in PAI-1 mutants with a highly accelerated conversion of active to latent forms. We can therefore conclude that the pronounced positive charge in the turn connecting s4C with s3C is of the highest importance for the functional stability of PAI-1.

【 授权许可】

Unknown