Chemotherapeutic activity of synthetic antimicrobial peptides: correlation between chemotherapeutic activity and neutrophil‐activating activity

FEBS Letters

Nakajima, Yuki¹  Natori, Shunji¹  Kanegasaki, Shiro²  Cho, Jang-hyun¹  Alvarez-Bravo, Juan¹  Homma, Ko-ichi¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108, Japan

关键词: Antibacterial peptide;    Chemotherapy;    Infection;    MRSA;    Neutrophil;    Superoxide;   

DOI  :  10.1016/S0014-5793(97)01101-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chemotherapeutic activity of three synthetic antibacterial peptides was investigated. KLKLLLLLKLK-NH₂ and its d-enantiomer showed significant chemotherapeutic activity in MRSA-infected mice, whereas KLKLLLKLK-NH₂, which showed the highest antibacterial activity among them in vitro, was found to have almost no ability to prevent MRSA infection. These results suggest that the antibacterial activity of peptides assessed in vitro does not necessarily correlate with their chemotherapeutic activity. We found that KLKLLLLLKLK-NH₂ and its d-enantiomer, but not KLKLLLKLK-NH₂, have the ability to activate human neutrophils to produce superoxide, suggesting that the prevention of MRSA infection by these peptides is not simply due to their direct bactericidal activity but to augmentation of the systemic defense mechanism mediated by neutrophils.

【 授权许可】

Unknown