α‐Tocopherol does not inhibit hypochlorite‐induced oxidation of apolipoprotein B‐100 of low‐density lipoprotein

FEBS Letters

Hazell, Linda J¹  Stocker, Roland¹ 

[1] The Heart Research Institute, 145 Missenden Rd, Camperdown, NSW 2050, Australia

关键词: Vitamin E;    Low-density lipoprotein;    Hypochlorous acid;    Protein oxidation;    Atherosclerosis;    AAPH;    2;    2′-azobis(2-amidinopropane) hydrochloride;    apo B-100;    apolipoprotein B-100;    CE-O(O)H;    the sum of cholesteryl ester hydroperoxides and cholesteryl ester hydroxides;    DMSO;    dimethyl sulphoxide;    EDTA;    ethylenediamine tetraacetic acid;    HOCl;    an equilibrium mixture of hypochlorous acid and hypochlorite at pH 7.4;    LDL;    low-density lipoprotein;    REM;    relative electrophoretic mobility;    α-TOH;    α-tocopherol;   

DOI  :  10.1016/S0014-5793(97)01066-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amount of α-tocopherol (α-TOH) can dramatically alter the extent of radical-induced oxidation of low density lipoprotein (LDL) lipids, a process generally thought to be important in atherogenesis. However, LDL with atherogenic features can also be formed in vitro by exposure to the strong non-radical oxidant hypochlorite (HOCl), which preferentially oxidises LDL apolipoprotein B-100. Here we show that varying LDL content of α-TOH by vitamin supplementation or depletion has no effect on the extent of HOCl-induced oxidation of apolipoprotein B-100 as measured by the loss of lysine and tryptophan residues, and the alteration in relative electrophoretic mobility of the lipoprotein particle.

【 授权许可】

Unknown