Structure of genes for dermaseptins B, antimicrobial peptides from frog skin

FEBS Letters

Nicolas, Pierre¹  Vouille, Véronique¹  Amiche, Mohamed¹ 

[1] Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, Université Paris 7, 2 Place Jussieu, 75251 Paris Cedex 01, France

关键词: Antimicrobial peptide;    Frog skin;    Gene structure;    Dermaseptin;    Opioid peptide;    bp;    base pairs;    nt;    nucleotide;    DRS;    dermaseptin;    cDNA;    complementary DNA;    kb;    kilobases;    TATA;    thymine–adenine–thymine–adenine;    UTR;    untranslated region;    SSC;    standard sodium citrate;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(97)00972-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned the genes of two members of the dermaseptin family, broad-spectrum antimicrobial peptides isolated from the skin of the arboreal frog Phyllomedusa bicolor. The dermaseptin gene Drg2 has a 2-exon coding structure interrupted by a small 137-bp intron, wherein exon 1 encoded a 22-residue hydrophobic signal peptide and the first three amino acids of the acidic propiece; exon 2 contained the 18 additional acidic residues of the propiece plus a typical prohormone processing signal Lys–Arg and a 32-residue dermaseptin progenitor sequence. The dermaseptin genes Drg2 and Drg1g2 have conserved sequences at both untranslated ends and in the first and second coding exons. In contrast, Drg1g2 comprises a third coding exon for a short version of the acidic propiece and a second dermaseptin progenitor sequence. Structural conservation between the two genes suggests that Drg1g2 arose recently from an ancestral Drg2-like gene through amplification of part of the second coding exon and 3′-untranslated region. Analysis of the cDNAs coding precursors for several frog skin peptides of highly different structures and activities demonstrates that the signal peptides and part of the acidic propieces are encoded by conserved nucleotides encompassed by the first coding exon of the dermaseptin genes. The organization of the genes that belong to this family, with the signal peptide and the progenitor sequence on separate exons, permits strikingly different peptides to be directed into the secretory pathway. The recruitment of such a homologous `secretory' exon by otherwise non-homologous genes may have been an early event in the evolution of amphibian.

【 授权许可】

Unknown