Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin

FEBS Letters

Escribano, Julio²  Ortego, Javier¹  Coca-Prados, Miguel¹ 

[1] Department of Ophthalmology and Visual Science, Yale University School of Medicine, 330 Cedar St., New Haven, CT 06510, USA;Laboratorio de Biotecnologı́a, Instituto de Desarrollo Regional, Universidad de Castilla-La Mancha, 02071 Albacete, Spain

关键词: Subtracted cDNAs;    Human ocular ciliary body;    TIGR gene;    Myosin;    Olfactomedin;    mRNA expression;   

DOI  :  10.1016/S0014-5793(97)00934-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open angle glaucoma [Science 275 (1997) 668–670]. Several structural features of the deduced human protein have been noted: a cleavable N-terminal signal peptide, a periodic repetition at the N-terminus of leucine and arginine residues at every seventh and eleven position respectively in helix conformation (leucine zipper-like motif) exhibiting homology with myosin, and with olfactomedin in the C-terminus. The mRNA for TIGR is abundantly expressed in the ciliary body, iris, heart and skeletal muscle.

【 授权许可】

Unknown