Functional analysis of a rat sodium channel carrying a mutation for insect knock‐down resistance (kdr) to pyrethroids

FEBS Letters

Devonshire, A.L²  Usherwood, P.N.R¹  Vais, H¹  Eldursi, N¹  Hick, C.A²  Williamson, M.S² 

[1] Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK;IACR-Rothamsted, Harpenden Herts AL5 2JQ, UK

关键词: Sodium channel;    Xenopus oocyte;    Rat brain α-subunit;    Housefly para-subunit;    Knock-down resistance (kdr);    Permethrin;   

DOI  :  10.1016/S0014-5793(97)00931-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pyrethroid insensitivity in resistant (kdr) insects has been correlated with a leucine to phenylalanine replacement in the S6 transmembrane segment of domain II of the axonal sodium channel α(para)-subunit. An α-subunit of rat brain type II sodium channel containing this mutation has been expressed and its sensitivity to permethrin compared with that of the wild-type channel. The steady-state activation curve of the mutant was shifted 14 mV in the depolarizing direction. We propose that an equivalent shift of the sodium current activation curve in kdr insects could account for their low sensitivity to permethrin toxicity.

【 授权许可】

Unknown