Modulation of the skeletal muscle sodium channel α‐subunit by the β1 ‐subunit

FEBS Letters

Weigl, Lukas¹  Wallner, Martin¹  Meera, Pratap¹  Lotan, Ilana² 

[1] Institut für Medizinische Physik und Biophysik, Universitaet Graz, Graz, Austria;Department of Physiology and Pharmacology, Sackler Medical School, University of Tel Aviv, Tel Aviv, Israel

关键词: Sodium channel;    α-Subunit;    β1-Subunit;    Xenopus oocyte;    Expression;    Translational control;    β1β1-subunit of the sodium channel;    αμI;    rat skeletal muscle sodium channel α-subunit isoforms;    αIIa;    IIa rat brain sodium channel α-subunit;    UTR;    untranslated region;   

DOI  :  10.1016/0014-5793(93)80871-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Co-expression of cloned sodium channel β₁ -subunit with the rat skeletal muscle-subunit (α_μI) accelerated the macroscopic current decay, enhanced the current amplitude, shifted the steady state inactivation curve to more negative potentials and decreased the time required for complete recovery from inactivation. Sodium channels expressed from skeletal muscle mRNA showed a similar behaviour to that observed from , indicating that β₁ restores ‘physiological’ behaviour. Northern blot analysis revealed that the Na⁺ channel β₁-subunit is present in high abundance (about 0.1%) in rat heart, brain and skeletal muscle, and the hybridization with untranslated region of the ‘brain’ β₁ cDNA to skeletal muscle and heart mRNA indicated that the diffferent Na ⁺ channel α-subunits in brain, skeletal muscle and heart may share a common β₁ -subunit.

【 授权许可】

Unknown