An AU at the first base pair of helix 3 elevates the catalytic activity of hepatitis delta virus ribozymes

FEBS Letters

Ping, Yueh-Hsin²  Wu, Huey-Nan²  Huang, Zhi-Shun¹ 

[1] Graduate Institute of Biotechnology, Chinese Culture University, Taipei, Taiwan;Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan

关键词: Ribozyme;    Hepatitis delta virus (HDV) ribozyme;    cis-Cleavage;    trans-Cleavage;   

DOI  :  10.1016/S0014-5793(97)00902-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mutational analysis of the helix 3 (H3) region of hepatitis delta virus (HDV) ribozymes disclosed that an AU at the first base pair of H3 elevates the catalytic activity of various cis- and trans-acting HDV ribozymes. A GC to AU substitution at this position of H3, which is located at the junction of three of the four helices of the pseudoknot-like structure model, altered the structure of HDV ribozymes. This substitution in the H3 did not change the independence of the cleavage rate to pH nor the sensitivity to formamide treatment of the ribozymes.

【 授权许可】

Unknown