FEBS Letters | |
Catalytic activities of hammerhead ribozymes with a triterpenoid linker instead of stem/loop II | |
Amontov, Sergei1  Saito, Isao2  Hatano, Ken2  Taira, Kazunari1  Sugiyama, Hiroshi2  | |
[1] National Institute of Bioscience and Human Technology, AIST, MITI, 1-1 Higashi, Tsukuba Science City 305, Japan;Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-01, Japan | |
关键词: Ribozyme; Hammerhead; Dimer; Triterpenoid; Kinetics; | |
DOI : 10.1016/0014-5793(96)00814-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem/loop II. In a previous study we demonstrated that a minizyme with high-level activity forms a dimeric structure with a common stem II (Amontov and Taira, J. Am. Chem. Soc., 118 (1996) 1624–1628). As a continuation of this study, we recently examined whether a short oligonucleotide linker at stem/loop II could be replaced by a triterpenoid linker and whether the resulting minizymes with bulky hydrophobic groups would form dimeric structures. In contrast to the minizyme with high-level activity that we characterized in the previous study, minizymes that contained a triterpenoid group had low cleavage activities. The nature of the dependence of the activity on the concentration of ribozyme revealed that these minizymes with a triterpenoid group do not form dimeric structures. Thus, the low activities of these structures can be attributed to their failure to form dimers.
【 授权许可】
Unknown
【 预 览 】
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