FEBS Letters | |
Identification of a 48‐kDa prenylated protein that associates with microtubules as 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase in FRTL‐5 cells | |
Bifulco, Maurizio1  Wolff, J2  Laezza, Chiara1  | |
[1] CEOS/CNR and Dipartimento di Biologia e Patologia Cellulare e Molecolare `L. Califano', Università `Federico II', via S. Pansini 5, 80131 Napoli, Italy;Laboratory of Biochemical Pharmacology, NIDDK, NIH, Bethesda, MD20892, USA | |
关键词: 2′; 3′-Cyclic nucleotide 3′-phosphodiesterase; Microtubule-associated proteins; Prenylation; FRTL-5 thyroid cells; CNP1; 2; 2′; 3′-cyclic nucleotide 3′-phosphodiesterase 1 and 2; MAP; microtubule-associated proteins; MVA; mevalonate; pCNP7; CNP1 cDNA; PBS; phosphate buffered saline; Pipes; piperazine N; N-bis(2-ethane sulfonic acid); PMSF; phenylmethyl sulfonyl fluoride; DTT; dithiothreitol; | |
DOI : 10.1016/S0014-5793(97)00924-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In an effort to study the nature of tubulin attachment to membranes, we have previously observed that after blocking prenylation in FRTL-5 thyroid cells, the microtubules become disconnected from the plasma membrane region [Bifulco M. et al. (1983) J. Cell. Physiol. 155, 340–348]. In this study we show that several [3H]mevalonate labeled proteins in FRTL-5 cells associate with membrane and cytoskeleton and, among these, we describe the presence of a 48-kDa prenylated protein, identified by immunoprecipitation as 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP), that associates with microtubules. This latter association persists through several polymerization/depolymerization cycles, whereas other prenylated proteins are lost. It is suggested that CNP can be a novel microtubule-associated protein (MAP) and a promising candidate as a membrane anchor for microtubules.
【 授权许可】
Unknown
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