Differential display PCR reveals increased expression of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase by lithium

FEBS Letters

Wang, Jun-Feng¹  Young, L.Trevor¹ 

[1] Departments of Psychiatry and Biomedical Sciences, McMaster University, 1200 Main St. W. Room 3G57, Hamilton, Ont. L8N 3Z5, Canada

关键词: Differential display PCR;    Lithium;    2′;    3′-Cyclic nucleotide 3′-phosphodiesterase;    Bipolar disorder;    Gene expression;    DD-PCR;    differential display polymerase chain reaction;    CNPase;    2′;    3′-cyclic nucleotide 3′-phosphodiesterase;    LiRG;    lithium-regulated gene;    MBP;    myelin basic protein;    G-protein;    GTP binding protein;    PKC;    protein kinase C;    CNS;    central nervous system;    BD;    bipolar affective disorder;   

DOI  :  10.1016/0014-5793(96)00433-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Differential display PCR was used to study the effects of lithium on gene expression. Four candidate genes were isolated and verified by Northern hybridization after 1 week treatment of C6 glioma cells with therapeutically relevant concentrations of LiCl (1 mM). Sequencing analysis revealed three previously unidentified cDNA fragments in addition to a sequence with 99% homology with the cDNA for 2′,3′-cyclic nucleotide 3′-phosphodiesterase type II (CNPaseII). Since CNPaseII is important in myelinogenesis and possibly neuronal growth and repair, the present findings suggest that lithium treatment may regulate these processes.

【 授权许可】

Unknown