Detection of secretory phospholipase A2s related but not identical to type IIA isozyme in cultured mast cells

FEBS Letters

Kambe, Terumi¹  Murakami, Makoto¹  Sawada, Hisashi¹  Tada, Kinji¹  Shimbara, Satoko¹  Kudo, Ichiro¹ 

[1] Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142, Japan

关键词: Phospholipase A2;    Mast cell;    Prostaglandin D2;    Cytokine (mouse);    BMMC;    bone marrow-derived mast cells;    cPLA2;    cytosolic phospholipase A2;    sPLA2;    secretory phospholipase A2;    COX;    cyclooxygenase;    FcεRI;    Fcε receptor I;    PGD2;    prostaglandin D2;    IL;    interleukin;    KL;    c-kit ligand;    TNP;    trinitrophenyl;   

DOI  :  10.1016/S0014-5793(97)00916-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously reported that BALB/cJ mouse-derived bone marrow-derived mast cells (BMMC) exhibited two sequential phases of prostaglandin D₂ (PGD₂) generation in response to Fc_ε receptor I (Fc_εRI) crosslinking and cytokine stimulation, the late phase of which was suppressed by an antibody raised against type IIA secretory phospholipase A₂ (sPLA₂). Here we report that BMMC derived from C57BL/6J mice, which are genetically deficient in type IIA sPLA₂, display both immediate and delayed PGD₂ generation normally. Lysates of C57BL/6J-derived BMMC contained a Ca²⁺-dependent PLA₂ that was absorbed to a column conjugated with anti-type IIA sPLA₂ antibody and had a similar molecular mass of 14 kDa, as assessed by immunoblotting. Therefore we speculate that a sPLA₂ similar to, but distinct from, type IIA sPLA₂ would compensate for type IIA sPLA₂ deficiency in C57BL/6J-derived BMMC. We found that the two type IIA-related sPLA₂ family members, type V and type IIC sPLA₂s, were expressed in BMMC as well as in rat mastocytoma RBL-2H3 cells.

【 授权许可】

Unknown