Eosinophils in human oral squamous carcinoma; role of prostaglandin D2

Journal of Inflammation

Research

W Tim McGaw¹  Donald Yu¹  Redwan Moqbel²  Lakshmi Puttagunta³  Adrian Sim³  Sibina Fisher⁴  Darryl J Adamko⁵  Charlie Tang⁶  Yingqi Wu⁶  Caroline Ethier⁶  Lisa Cameron⁶  Francis Davoine⁷ 

[1] Department of Dentistry, University of Alberta, Edmonton, Alberta, Canada;Department of Immunology, University of Manitoba, Winnipeg, Alberta, Canada;Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada;Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada;Department of Paediatrics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;Pulmonary Research Group, University of Alberta, 559 Heritage Medical Research Centre, T6G 2S2, Edmonton, Alberta, Canada;Pulmonary Research Group, University of Alberta, 559 Heritage Medical Research Centre, T6G 2S2, Edmonton, Alberta, Canada;Campus-Saint-Jean, University of Alberta, 8406 Marie-Anne-Gaboury Street, T6C 4G9, Edmonton, Alberta, Canada;

关键词: Eosinophils;    Oral squamous carcinoma;    Prostaglandin D2;    HQL-79;    Transmigration;   

DOI  :  10.1186/1476-9255-10-4

 received in 2012-02-17, accepted in 2013-01-28,  发布年份 2013

来源: Springer

【 摘 要 】

Eosinophils are often predominant inflammatory leukocytes infiltrating oral squamous carcinoma (OSC) sites. Prostaglandins are secreted by oral carcinomas and may be involved in eosinophil infiltration. The objective of this study was to determine the factors contributing to eosinophil migration and potential anti-neoplastic effects on OSC. Eosinophil degranulation was evaluated by measuring release of eosinophil peroxidase (EPO). Eosinophil chemotaxis towards OSC cells was assessed using artificial basement membrane. Eosinophil infiltration was prominent within the tissue surrounding the OSC tumor mass. We observed growth inhibition of the OSC cell line, SCC-9, during co-culture with human eosinophils, in vitro, which correlated with EPO activity that possesses growth inhibitory activity. The PGD2 synthase inhibitor, HQL-79, abrogated migration towards SCC-9. Our data suggest that OSC-derived PGD2 may play an important role via CRTH2 (the PGD2 receptor on eosinophils) in eosinophil recruitment and subsequent anti-tumor activity through the action of eosinophil cationic proteins.

【 授权许可】

CC BY   
© Davoine et al; licensee BioMed Central Ltd. 2013