Mutational analysis of human prothymosin α reveals a bipartite nuclear localization signal

FEBS Letters

Karger, Elena M.²  Pavlov, Nickolay A.²  Evstafieva, Alexandra G.²  Zolotukhin, Andrei S.¹  Vartapetian, Andrey B.²  Chichkova, Nina V.²  Rubtsov, Yuri P.²  Felber, Barbara K.¹  Vorobjev, Ivan A.² 

[1] ABL-Basic Research Program, National Cancer Institute-FCRDC, Frederick, MD 21701, USA;Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia

关键词: Prothymosin α;    PCR-based mutagenesis;    Cell growth inhibition;    Nuclear localization signal;    Saccharomyces cerevisiae;    ProTα;    prothymosin α;    bp;    base pair(s);    CMV;    cytomegalovirus;    GFP;    green fluorescent protein;    NLS;    nuclear localization signal;    PCR;    polymerase chain reaction;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(97)00824-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutants of human prothymosin α with impaired ability to inhibit yeast Saccharomyces cerevisiae. cerevisiae cell growth were characterized. Two types of prothymosin α-inactivating mutations were observed. Mutations that belong to the first type compromised the nuclear entry of prothymosin α by affecting its nuclear localization signal. Analysis of subcellular distribution of GFP-prothymosin α fusions revealed a bipartite nuclear localization signal that is both necessary and sufficient for nuclear import of the protein in human cells. Mutations of the second type abrogated the inhibitory action of prothymosin α through an unknown mechanism, without influencing the nuclear import of the protein.

【 授权许可】

Unknown