HIV‐1 envelope protein, gp120, has no effects on inositol phosphate production and metabolism in the Jurkat T‐cell line either in the presence or absence of receptor stimulation

FEBS Letters

Shears, Stephen B¹  Sumner, Martina T¹ 

[1] Inositol Lipid Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, P.O. Box 12233, National Institutes of Health, Research Triangle Park, NC 27709, USA

关键词: HIV envelope glycoprotein;    Signal transduction;    CD3/TCR complex;    Inositol phosphate metabolism;   

DOI  :  10.1016/S0014-5793(97)00880-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4⁺ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol polyphosphate 5-phosphatase. Treatment of cells with up to 10 μg/ml gp120 from between 10 min and 24 h was without effect upon inositol phosphate turnover in both basal cells, and in C305 and OKT3 stimulated cells. This is the first report that biologically competent gp120 does not affect any aspect of inositol phosphate turnover in either basal or receptor-activated lymphocytes.

【 授权许可】

Unknown