Functional cloning of a cDNA encoding Mei2‐like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant

FEBS Letters

Shimoda, Chikashi³  Yamamoto, Masayuki⁴  Ishida, Chika¹  Hirayama, Takashi²  Kuromori, Takashi⁴  Shinozaki, Kazuo²  Obata, Shusei¹  Ohto, Chikara¹ 

[1] Bio Research Laboratory, Toyota Motor Corporation, 1 Toyota-cho, Toyota, Aichi 471-71, Japan;Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1, Koyadai, Tsukuba, Ibaraki 305, Japan;Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan;Division of Cell Proliferation, National Institute for Basic Biology, Okazaki, Aichi 444, Japan

关键词: Arabidopsis thaliana;    Schizosaccharomyces pombe;    Trans-complementation;    Meiosis induction;    RNA-recognition motif;   

DOI  :  10.1016/S0014-5793(97)00871-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To isolate Arabidopsis cDNAs that encode signal transducers and components involved in the regulation of meiosis, a trans-complementation analysis was performed using a Schizosaccharomyces pombe meiosis-defective mutant in which the genes for pheromone receptors were disabled. One cDNA obtained in this screening encodes a polypeptide, named AML1, that shows significant similarity to S. pombe Mei2 protein and has three putative RNA-recognition motifs like as Mei2. Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed.

【 授权许可】

Unknown