The receptor for urokinase‐type plasminogen activator is expressed during mouse spermatogenesis

FEBS Letters

Vassalli, Jean-Dominique¹  Zhou, Hongming¹ 

[1] Department of Morphology, University of Geneva Medical School, Rue Michel-Servet 1, 1211 Geneva, Switzerland

关键词: uPA receptor;    Germ cell;    Spermatozoa;    Local proteolysis;    uPA;    urokinase-type plasminogen activator;    uPAR;    uPA receptor;    tPA;    tissue-type plasminogen activator;    ΔG-uPA;    receptor-binding domain-deleted uPA;    FCS;    fetal calf serum;    SGCC;    Sertoli-germ cells co-culture;    SDS-PAGE;    sodium dodecylsulfate–polyacrylamide gel electrophoresis;    BCIP;    5-bromo-4-chloro-3-indollyl-phosphate;    NBT;    4-nitro blue tetrazolium;   

DOI  :  10.1016/S0014-5793(97)00861-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Urokinase-type plasminogen activator (uPA) binds to a plasma membrane receptor (uPAR) that localizes plasmin generation to the cell environment. Mouse spermatozoa have surface-bound uPA, which appears to be acquired from genital tract secretions at ejaculation. We determined the presence of uPAR mRNA in spermatogenic cells and their uPA-binding activity. Northern blot and in situ hybridization demonstrated the presence of uPAR mRNA in germ cells. Binding of uPA, but not of a mutant enzyme lacking the receptor-binding domain, indicated the presence of uPAR on spermatids and spermatozoa. The uPAR and/or receptor-bound uPA may be involved in spermatogenesis, spermatozoa maturation or fertilization.

【 授权许可】

Unknown