Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction

FEBS Letters

Anraku, Yasuhiro¹  Kawasaki, Masato¹  Ohya, Yoshikazu¹  Satow, Yoshinori² 

[1]Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

[2]Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Intramolecular reaction;    Protease inhibitor;    Protein splicing;    Refolding;    Vma1 protozyme;    DTT;    dithiothreitol;    EDTA;    ethylenediaminetetraacetic acid;    PAGE;    polyacrylamide gel electrophoresis;    VDE;    VMA1-derived endonuclease;    Vma1p;    the 70 kDa catalytic subunit of vacuolar H+-ATPase;    Vma1 protozyme;    the 120 kDa VMA1 gene translation product;   

DOI  :  10.1016/S0014-5793(97)00850-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein splicing is an autocatalytic reaction of a single polypeptide in which a spliced intervening sequence is excised out and the two external regions are ligated with the peptide bond to yield two mature proteins. We examined the reaction mechanism using a folding-dependent in vitro protein splicing system. Protein splicing proceeds at an optimal pH of 7 and is an intramolecular reaction. The reaction is not inhibited by potential protease inhibitors, suggesting that its mechanism is different from those catalyzed by known proteases.

【 授权许可】

Unknown