Ion channels formed in planar lipid bilayers by Bacillus thuringiensis toxins in the presence of Manduca sexta midgut receptors

FEBS Letters

Lu, Yiang-Jiang²  Masson, Luke¹  Adang, Michael J²  Schwartz, Jean-Louis¹  Brousseau, Roland¹  Laprade, Raynald³  Söhnlein, Petra³ 

[1]Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montreal, Que. H4P 2R2, Canada

[2]Department of Entomology, University of Georgia, Athens, GA 30602, USA

[3]Groupe de recherche en transport membranaire, Université de Montréal, CP 6128, succ. Centre Ville, Montreal, Que. H3C 3J7, Canada

关键词: Ion channel;    Receptor;    Bacterial toxin;    Planar lipid bilayer;    Surface plasmon resonance;    Bacillus thuringiensis;   

DOI  :  10.1016/S0014-5793(97)00801-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A purified, GPI-linked receptor complex isolated from Manduca sexta midgut epithelial cells was reconstituted in planar lipid bilayers. CryIAa, CryIAc and CryIC, three Bacillus thuringiensis insecticidal proteins, formed channels at much lower doses (0.33–1.7 nM) than in receptor-free membranes. The non-toxic protein CryIB also formed channels, but at doses exceeding 80 nM. The channels of CryIAc, the most potent toxin against M. sexta, rectified the passage of cations. All other toxin channels displayed linear current–voltage relationships. Therefore, reconstituted Cry receptors catalyzed channel formation in phospholipid membranes and, in two cases, were involved in altering their biophysical properties.

【 授权许可】

Unknown