MDR‐1 gene expression is a minor factor in determining the multidrug resistance phenotype of MCF7/ADR and KB‐V1 cells

FEBS Letters

Kim, Hwan Mook²  Park, Sun Mi²  Kim, Moon Soon²  Hong, Dong Ho²  Oh, Goo Taeg²  Han, Sang Bae²  Kang, Jong Seong¹ 

[1] Department of Pharmacy, Chungnam National University, Chungnam, South Korea;Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305–600, South Korea

关键词: MDR gene expression;    KB-V1;    KB-3–1;    MCF7;    MCF7/ADR;    Influx;    Efflux;    MDR;    multidrug resistance;    R-123;    rhodamine 123;    ADR;    adriamycin;    VBL;    vinblastine;    VP;    verapamil;    SRB;    sulforhodamine B;   

DOI  :  10.1016/S0014-5793(97)00777-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3–1 and MCF7. The drug efflux rate of KB-V1 was the same as KB-3–1 although the MDR-1 gene was expressed in only the resistant cell. The higher intracellular drug concentration of KB-3–1 than KB-V1 was due to the large drug influx. In the case of MCF7 and MCF7/ADR, the influx and efflux of the drug had nearly the same pattern and drug efflux was not affected by verapamil. The amount of ATP, cofactor of drug pumping activity of P-glycoprotein, was not changed by the resistance. These observations suggested that drug efflux mediated by MDR-1 gene expression was not a major determining factor of drug resistance in the present cell systems, and that the drug resistance could be derived from the change in drug uptake and other mechanisms.

【 授权许可】

Unknown