Acylphosphate formation by the Menkes copper ATPase

FEBS Letters

Camakaris, James¹  Solioz, Marc² 

[1]Department of Genetics, Melbourne University, 3052 Parkville, Victoria, Australia

[2]Department of Clinical Pharmacology, University of Berne, Murtenstrasse 35, 3010 Berne, Switzerland

关键词: Menkes disease;    P-type ATPase;    Copper;    Phosphorylation;    Copper resistance;    (Hamster);    CHO;    Chinese hamster ovary;    K1;    control CHO cell line;    CUR3;    copper-resistant CHO cell line;    CUR3−;    CUR3 cells grown without added copper;    CUR3+;    CUR3 cells grown in 500 μM CuSO4;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(97)00770-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Menkes ATPase is the product of the MNK gene, defective in some inherited human disorders of copper metabolism. We here show the formation of an acylphosphate intermediate by the murine MNK homologue in membranes from normal and copper resistant Chinese hamster ovary cells. In the latter, fivefold higher levels of acylphosphate were formed. Challenging these cells with copper, which induces relocation of the MNK ATPase from the trans-Golgi network to the plasma membrane, did not influence acylphosphate formation. The kinetics of phosphorylation, metal dependence, and sensitivity to inhibitors were investigated. The results show that the MNK ATPase is an active P-type ATPase and provide a direct functional test for this enzyme.

【 授权许可】

Unknown