FEBS Letters | |
Channel activators regulate ATP‐sensitive potassium channel (KIR6.1) expression in chick cardiomyocytes | |
Lu, Changwan1  Halvorsen, Stanley W1  | |
[1] Department of Biochemical Pharmacology, 447 Hochstetter Hall, Box 601200, State University of New York at Buffalo, Amherst, NY 14260, USA | |
关键词: Potassium channel; Sulfonylurea; mRNA regulation; Cardiac ion channel; Pinacidil; ATP sensitive; KATP; ATP-sensitive potassium channel; GIRK; G-protein-coupled inwardly rectifying potassium channel; Kir; inwardly rectifying potassium channel; RT-PCR; reverse transcription-polymerase chain reaction; | |
DOI : 10.1016/S0014-5793(97)00760-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
ATP-sensitive potassium channels (KATP) are widely expressed and yet little is known about the mechanisms regulating their expression. Here we report that expression of chick heart Kir6.1 is regulated by channel activators. Activation of KATP with either ATP depletion or pinacidil, up-regulated Kir6.1 mRNA 1.8- to 2.4-fold in cultured ventricular myocytes as measured by competitive PCR. Pinacidil treatment also increased Kir6.1 protein as detected using an antibody to Kir6.1. Glibenclamide, a KATP inhibitor, completely blocked the pinacidil-induced increase in Kir6.1 levels. It appears that Kir6.1 is up-regulated by an unknown signal transduction pathway initiated by KATP opening.
【 授权许可】
Unknown
【 预 览 】
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RO201912020304647ZK.pdf | 561KB | download |