Channel activators regulate ATP‐sensitive potassium channel (KIR6.1) expression in chick cardiomyocytes

FEBS Letters

Lu, Changwan¹  Halvorsen, Stanley W¹ 

[1] Department of Biochemical Pharmacology, 447 Hochstetter Hall, Box 601200, State University of New York at Buffalo, Amherst, NY 14260, USA

关键词: Potassium channel;    Sulfonylurea;    mRNA regulation;    Cardiac ion channel;    Pinacidil;    ATP sensitive;    KATP;    ATP-sensitive potassium channel;    GIRK;    G-protein-coupled inwardly rectifying potassium channel;    Kir;    inwardly rectifying potassium channel;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)00760-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP-sensitive potassium channels (K_ATP) are widely expressed and yet little is known about the mechanisms regulating their expression. Here we report that expression of chick heart Kir6.1 is regulated by channel activators. Activation of K_ATP with either ATP depletion or pinacidil, up-regulated Kir6.1 mRNA 1.8- to 2.4-fold in cultured ventricular myocytes as measured by competitive PCR. Pinacidil treatment also increased Kir6.1 protein as detected using an antibody to Kir6.1. Glibenclamide, a K_ATP inhibitor, completely blocked the pinacidil-induced increase in Kir6.1 levels. It appears that Kir6.1 is up-regulated by an unknown signal transduction pathway initiated by K_ATP opening.

【 授权许可】

Unknown