RAP1‐like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors

FEBS Letters

Arnold, Rudolf¹  Schütze, Hans-Joachim¹  Münch, Karin¹  Simon, Babette¹  Merchant, Juanita L.²  Wattler, Frank¹  Suske, Guntram³ 

[1] Department of Internal Medicine, Division of Gastroenterology and Metabolism, Philipps-University, 35033 Marburg, Germany;Institute of Molecular Tumorbiology, Philipps-University, 35033 Marburg, Germany;Department of Internal Medicine and Physiology, University of Michigan, Howard Hughes Medical Institute, Ann Arbor, MI 48109, USA

关键词: Gastrin promoter;    Islet cell;    RAP1;    Sp-related transcription factor;   

DOI  :  10.1016/S0014-5793(97)00736-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1- and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.

【 授权许可】

Unknown