P2X7 purinoceptor expression in Xenopus oocytes is not sufficient to produce a pore‐forming P2Z‐like phenotype

FEBS Letters

Singer, Joshua J¹  Ugur, Mehmet¹  Drummond, Robert M¹  V. Walsh, John¹  Petrou, Steven¹ 

[1] Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655-0127, USA

关键词: P2 purinoceptor;    Pore formation;    Electrophysiology;    Oocyte expression;    BzATP;    2′- and 3′-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate;    NMDG+;    N-methyl-d-glucamine;    rP2X7;    rat P2X7;   

DOI  :  10.1016/S0014-5793(97)00700-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purinergic rP2X7 receptor expressed in a number of heterologous systems not only functions as a cation channel but also gives rise to a P2Z-like response, i.e. a reversible membrane permeabilization that allows the passage of molecules with molecular masses of ≥300 Da. We investigated the properties of rP2X7 receptors expressed in Xenopus oocytes. In two-electrode voltage-clamp experiments, ATP or BzATP caused inward currents that were abolished or greatly diminished when NMDG⁺ or choline⁺ replaced Na⁺ as the principal external cation. In fluorescent dye experiments, BzATP application did not result in entry of the fluorophore YO-PRO-1²⁺. Thus, rP2X7 expression in Xenopus oocytes does not by itself give rise to the pore-forming P2Z phenotype, suggesting that ancillary factors are involved.

【 授权许可】

Unknown