Evidence for a 65 kDa sulfonylurea receptor in rat pancreatic zymogen granule membranes

FEBS Letters

Anderie, Ines¹  Thévenod, Frank¹  Braun, Matthias¹ 

[1] II Department of Physiology, University of the Saarland, 66421 Homburg/Saar, Germany

关键词: KATP channel;    Sulfonylurea receptor;    Glibenclamide;    Secretory granule;    MDR1;    ABC;    ATP binding cassette;    CCCP;    carbonyl-cyanide m-chlorophenylhydrazone;    CFTR;    cystic fibrosis transmembrane conductance regulator;    KATP channel;    ATP-sensitive K+ channel;    Kir channel;    inwardly rectifying K+ channel;    mdr;    multidrug resistance P-glycoprotein;    SUR;    sulfonylurea receptor;    ZG;    zymogen granules;    ZGM;    zymogen granule membranes;   

DOI  :  10.1016/S0014-5793(97)00711-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat pancreatic zymogen granules (ZG), a K⁺ selective conductance which can be blocked by ATP has been characterized. Here we show that this pathway can be specifically blocked by glibenclamide. Using a rapid filtration assay, we also found specific binding of [³H]glibenclamide to a low-affinity site (K _d 5.6±1.1 μM) in rat pancreatic zymogen granule membranes (ZGM). In photoaffinity labeling experiments with [³H]glibenclamide, a 65±1.5 kDa polypeptide was specifically labeled. Previously, a ∼65 kDa mdr1 gene product has been demonstrated to be involved in the regulation of the K⁺ selective conductance of ZG. We conclude that this protein may be a subunit of, or associated with, a ZG K_ATP channel.

【 授权许可】

Unknown